Thermo Scientific Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates - 140654 (3 μm) (12-Well Plate)

Cultivate most cell types without matrix coating when using Thermo Scientific Nunc Polycarbonate Membrane Inserts in Multidishes. Features:

• Tissue culture-treated porous membrane for optimized cell attachment
• Three pore sizes: 0.4, 3, and 8 micron
• Pre-packed in sterile Nunclon Delta-treated multi-well plates
• Nontoxic and nonpyrogenic

Applications:

• Tumor migration and invasion assays
• Epithelial (e.g. Caco-2) and endothelial (e.g. brain microvascular endothelial cells) barriers assays
• Dermal/epidermal and epithelial tissue models (e.g. artificial skin model)
• Chemotaxis studies
• Co-culture

Warranty: 90 days

WARNING: Cancer – www.P65Warnings.ca.gov Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

General References:

1. Mardi A Crane-Godreau et.al (2005). CCL20/macrophage Inflammatory Protein 3alpha and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment With Lipopolysaccharide or Pam3Cys. PMID: 15618187
2. Rettinger CL et. al (2018). Quantitative Assessment of Retina Explant Viability in a Porcine Ex Vivo Neuroretina Model. PMID: 29924674
3. Mardi A Crane-Godreau et.al (2004). Effect of Escherichia coli and Lactobacillus rhamnosus on macrophage inflammatory protein 3 alpha, tumor necrosis factor alpha, and transforming growth factor beta release by polarized rat uterine epithelial cells in culture. PMID: 15039305
4. Soboll G et. al (2006). Effect of oestradiol on PAMP-mediated CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture. PMID: 16771853
5. Mardi A Crane-Godreau et. al (2009). Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells. PMID: 19966927
6. Wildemann B et. al (2005). Differences in the fusion and resorption activity of human osteoclasts after stimulation with different growth factors released from a polylactide carrier. PMID: 15477999
7. Sugita Y et. al (1997). Pigment epithelium-derived factor (PEDF) has direct on the metabolism and proliferation of microglia and indirect effects on astrocytes. PMID: 9335258
8. van Schilfgaarde M et. al (1999). Haemophilus influenzae localized in epithelial cell layers is shielded from antibiotics and antibody-mediated bactericidal activity. PMID: 10222210
9. Chung HC et. al (2014). Inhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference. PMID: 24138389
10. Ode A et. al (2010). Toward biomimetic materials in bone regeneration: functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components. PMID: 20878902