Restore™ Western Blot Stripping Buffer, Trial Size, 30 mL

Thermo Scientific™ Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. The reagent allows clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen. This allows blots to be stripped and reprobed with confidence.Highlights:Saves time – no need to re-run gels and blotsSaves costly sample – re-probe the membrane using the same target sampleEffective – formulation is more efficient at stripping antibodies than homemade buffersGentle – does not damage the target antigen during stripping allowing efficient reprobingOdor-free – no mercaptans means no acrid odorsEconomical – less expensive than other commercial stripping buffersReuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody; Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time.