Pierce™ Streptavidin Coated High Capacity Plates

Pierce Streptavidin High Binding Capacity (HBC) Plates are prepared with an exclusive coating method that yields a streptavidin-coated plate with four to five times the binding capacity of other commercially available polystyrene assay plates. Using a Pierce Streptavidin HBC Plate can result in an assay with a broader dynamic range and better linearity, leading to improved assay precision. The plates are offered in several plate formats, plate colors, and coating volumes.Thermo Scientific Pierce Streptavidin-Coated High-Capacity Plates are designed for the binding of biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.Pierce Streptavidin-coated plates are widely used for applications such as ELISAs and other assay protocols. These biotin-binding plates come with different capacities and sensitivities for maximum flexibility. For example, the high-binding-capacity plates (HBC) can be used for anti-therapeutic/anti-drug antibodies (ADA) screening for immunogenicity research and biologics development (1,2). Both regular and HBC plates have been used in aptamer assays for clinical and industrial needs, along with neurobiology research for therapeutics and prevention, amongst other applications (3–5).Features of Streptavidin-Coated High-Capacity Plates include:Unique plate-coating technology—results in high loading and binding capacityStreptavidin-coated option—plates have up to five times the binding capacity of typical platesBroader dynamic range—extends the quantitative range so there is less need for dilutionsSave time—pre-blocked plates to reduce the number of assay stepsFlexible assay formats—coated plates offered in 8-well strips to reduce wasteThe proprietary plate-coating technology used to manufacture these Pierce coated plates yields high-capacity plates with a wider detection limit than regular-binding-capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.ReferencesJ Immunol Methods. 2016 Apr;431:45-51J Immunol Methods. 2011 Feb 28;365(1-2):38-49Cancers (Basel). 2022 Oct 25;14(21):5222J Microbiol Biotechnol. 2013 Jun 28;23(6):878-84Acta Neuropathol Commun. 2018 May 31;6(1):43